The fMLF and WKYMVM peptides induce increases in intracellular calcium in neutrophils. Neutrophils loaded with Fura-2 were incubated at 37°C for 5 minutes. PBP10 (10-6 M final concentration) was present for the initial 5 minutes (B and D), and the cells were stimulated with fMLF (A and B; 10-7 M final concentration) or WKYMVM (C and D; 10-7 M final concentration). The changes in cytosolic Ca2+ levels were determined by measuring the fluorescence emitted at 509 nm following excitation at 340 nm and 380 nm. The results are presented as the ratios of the fluorescence intensities at 340 nm and 380 nm, and a representative experiment (of at least five repetitions) is shown.
Forsman and Dahlgren BMC Cell Biology 2010 11:52 doi:10.1186/1471-2121-11-52