Neutrophil release of superoxide anions induced by WKYMVM and fMLF, which are agonists for FPR2 and FPR1, respectively. Neutrophils were stimulated with fMLF (A; 10-7 M final concentration) or WKYMVM (B; 10-7 M final concentration) in the absence or presence of PBP10 (A and B; 10-6 M final concentration). The agonists were added at time 0, and the release of superoxide was followed using a chemiluminescence technique. Light emission was measured with (broken lines) or without (solid lines) the addition of PBP10. One representative of at least 100 experiments performed is shown. Abscissa, time of study (min); ordinate, superoxide production, given as light emission and expressed as cpm × 10-6.
Forsman and Dahlgren BMC Cell Biology 2010 11:52 doi:10.1186/1471-2121-11-52