Additional file 3.
Subculturing of Lyve1+ and Lyve1- cells after CD31/Lyve1 cell sorting. Early passage MLMVEC (passage 3) were FACS sorted (same cells as in supplementary figure 2) and separately cultured. Ten days after sub-culturing (passage 4) cells were studied again by FACS analysis with anti-Lyve1 antibodies. The CD31+/Lyve1+ cells indicated a stable high Lyve1 expression (a) whereas CD31+/Lyve- cells now expressed Lyve1 at a comparably high rate (c). One passage later (passage 5) some CD31+/Lyve+ cells were negative for Lyve1 (b) whereas CD31+/Lyve1- cells were getting even more positive for Lyve1 (d).
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Schniedermann et al. BMC Cell Biology 2010 11:50 doi:10.1186/1471-2121-11-50