Figure 3.

ICA results. Panels of ICA images showing (a) primary melanoma cell lines and (b) metastatic melanoma cell lines. Individual spots were acquired with 20× objective: each image shows a representative area of one spot of the indicated antibody. (c) Analysis of ICA results (see text for details) for primary melanoma cell lines; (d) analysis of ICA results for metastatic melanoma cell lines. Left panels refer to PIR normalized cytoplasmic staining; right panels refer to ShcA normalized cytoplasmic staining. ShcA = anti-ShcA antibody; PIR IP = immuno-purified anti-PIR antibody; PIR tot = anti-PIR total serum; P.I. = pre-immune serum.