Additional file 2.
HCCR-1 gene in embryonic kidney development. A. Detection of HCCR-1 protein in fetal 16-, 18-, 20-, postnatal 1-, 7-, 14-day and adult rat kidney tissue extracts. Total proteins were subjected to SDS-PAGE. HCCR-1 positive bands were revealed by ECL-Western blot detection kit. F and P denote fetal and postnatal, respectively. B. Immunohistochemical staining of 20-day-old fetal rat kidney. Immunostaining was confined to the collecting ducts. Magnification, × 42. C. Differential-interference contrast microscopy of 20-day-old fetal rat kidney illustrating HCCR-1 immunostaining in the basolateral plasma membrane of medullary collecting duct. Magnification, × 220.
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Ha et al. BMC Cell Biology 2010 11:49 doi:10.1186/1471-2121-11-49