Open Access Research article

Transdifferentiation-inducing HCCR-1 oncogene

Seon-Ah Ha1, Hyun K Kim1, JinAh Yoo1, SangHee Kim1, Seung M Shin1, Youn S Lee2, Soo Y Hur3, Yong W Kim3, Tae E Kim3, Yeun J Chung4, Shin S Jeun5, Dong W Kim6, Yong G Park7, Jin Kim8, Soon Y Shin9, Young H Lee9 and Jin W Kim13*

Author Affiliations

1 Molecular Genetic Laboratory, Catholic Medical Research Institute, The Catholic University of Korea, Seoul, Korea

2 Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea

3 Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea

4 Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Korea

5 Department of Neurosurgery, College of Medicine, The Catholic University of Korea, Seoul, Korea

6 Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea

7 Department of Biostatistics, College of Medicine, The Catholic University of Korea, Seoul, Korea

8 Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Korea

9 Department of Biomedical Science and Technology, Research Center for Transcription Control, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea

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BMC Cell Biology 2010, 11:49  doi:10.1186/1471-2121-11-49

Published: 30 June 2010

Additional files

Additional file 1:

Expression analysis of vimentin. (A, B) by immunofluorescence and epithelial markers such as E-cadherin, α-catenin, and β-catenin (C) by western blotting experiments. HEK-293 parental cells (A) and HEK-293 stable clones for HCCR-1 (B) were stained for anti-vimentin antibodies. In C, HEK-293 parental cells (lanes 1 and 2), HEK-293 stable cells for HCCR-1 (lanes 3 and 4), and HEK-293 cells transfected with empty vectors (lanes 5 and 6) were analyzed by antibodies against E-cadherin, α-catenin, and β-catenin.

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Additional file 2:

HCCR-1 gene in embryonic kidney development. A. Detection of HCCR-1 protein in fetal 16-, 18-, 20-, postnatal 1-, 7-, 14-day and adult rat kidney tissue extracts. Total proteins were subjected to SDS-PAGE. HCCR-1 positive bands were revealed by ECL-Western blot detection kit. F and P denote fetal and postnatal, respectively. B. Immunohistochemical staining of 20-day-old fetal rat kidney. Immunostaining was confined to the collecting ducts. Magnification, × 42. C. Differential-interference contrast microscopy of 20-day-old fetal rat kidney illustrating HCCR-1 immunostaining in the basolateral plasma membrane of medullary collecting duct. Magnification, × 220.

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