Ror1/2 mutant with the truncation of the ligand-binding and transmembrane domains are capable of nuclear transport. AGS and HR cells were transfected with the cytoplasmic part of Ror1/2 whose N-terminal was HcRed-tagged or with a vector control, and then analyzed by fluorescence confocal microscopy. The protein size of HcRed only is about 30 kDa; and the size of the HcRed-tagged Ror1/2 is about 85 kDa.
Tseng et al. BMC Cell Biology 2010 11:48 doi:10.1186/1471-2121-11-48