Subcellular localization of Swi6 in the kap95 mutant strain. Cells from exponentially growing cultures of the wild type (JCY710) and tetO 7:KAP95 (JCY637) strains expressing the HA-tagged version of Swi6, were incubated in the presence of 5 μg/mL doxycycline for 6 hours and assayed by indirect immunofluorescence. The HA indirect-fluorescence signals, the DAPI staining of DNA and DIC images are shown. No signal was detected in a control of the untagged W303-1a strain.
Taberner and Igual BMC Cell Biology 2010 11:47 doi:10.1186/1471-2121-11-47