Characterization of the terminal phenotype of tetO 7:KAP95 mutant strain. Exponentially growing cells of the wild type (W303-1a) and tetO 7:KAP95 (JCY635) strains were incubated in the presence of 5 μg/mL doxycycline for 6 hours. (A) DIC and DAPI staining of DNA images. Graph shows distribution of cells based on the bud size. (B) FACS analysis of DNA content. (C) Distribution of large budded cells based on number and location of nuclei. (D) Exponentially growing cells of the cdc15 and cdc15 tetO 7:KAP95 (JCY1543) strains were incubated in the presence of 5 μg/mL doxycycline at 37° for 4 hours. After transfer to 25°, cell morphology and nuclei number were analyzed at the indicated times (see text). (E) Cell morphology and nuclei number were analyzed in the samples of the cdc15 tetO 7:KAP95 (JCY1543) strain at 0 and 120 min after digestion of cell wall with zymolyase.
Taberner and Igual BMC Cell Biology 2010 11:47 doi:10.1186/1471-2121-11-47