NF-κB activation induced by exogenously added S1P is enhanced by S1P produced by the cells. Cells transfected with control siRNA or SK1 siRNA were treated with the indicated concentrations of S1P for 30 minutes and were analyzed for active NF-κB (p65) using ELISA. The panel to the right shows representative western blots of control and SphK1 siRNA treated cells. The bars denote the mean ± SEM of at least three independent experiments (*, p < 0.05).
Blom et al. BMC Cell Biology 2010 11:45 doi:10.1186/1471-2121-11-45