Table 1

Stable binding of Ran with RCC1 regulates chromatin binding of RCC1 through the N-terminal tail in live cells.

Constructs

Mean FRAP half-time ± SD (s)

Mean mobile fraction ± SD

n


GFP + mCherry

0.08 ± 0.09

0.92 ± 0.06

19

GFP + mCherry-RanWT

0.08 ± 0.08

0.89 ± 0.04

14

GFP + mCherry-RanT24N

0.09 ± 0.09

0.91 ± 0.06

17


RCC1α-GFP + mCherry

2.93 ± 0.56

0.91 ± 0.05

21

RCC1α-GFP + mCherry-RanWT

2.85 ± 0.63

0.91 ± 0.04

26

RCC1α-GFP + mCherry-RanT24N

3.69 ± 0.58

0.87 ± 0.05

21


Δ27RCC1-GFP + mCherry

0.81 ± 0.21

0.94 ± 0.05

23

Δ27RCC1-GFP + mCherry-RanWT

0.87 ± 0.27

0.95 ± 0.04

23

Δ27RCC1-GFP + mCherry-RanT24N

0.87 ± 0.28

0.94 ± 0.04

26


RCC1αK4Q-GFP + mCherry

2.19 ± 0.44

0.90 ± 0.06

27

RCC1αK4Q-GFP + mCherry-RanWT

1.82 ± 0.54

0.90 ± 0.06

26

RCC1αK4Q-GFP + mCherry-RanT24N

2.19 ± 0.63

0.90 ± 0.07

30


RCC1αD182A-GFP + mCherry

1.51 ± 0.39

0.93 ± 0.04

20

RCC1αD182A-GFP + mCherry-RanWT

1.30 ± 0.54

0.94 ± 0.05

10

RCC1αD182A-GFP + mCherry-RanT24N

2.63 ± 0.62

0.87 ± 0.05

12


Fluorescence recovery after photobleaching (FRAP) data derived from live U2OS cells co-expressing GFP, RCC1α-GFP, Δ27RCC1-GFP, RCC1α K4Q-GFP, RCC1αD182A. The table summarises the mean +/- standard deviation (SD) of FRAP half-time and mobile fraction of each of the GFP fusion constructs in the nuclei of cells that also expressed mCherry, mCherry-RanWT or mCherry-RanT24N. The number of cells used in each case is given (n).

Hitakomate et al. BMC Cell Biology 2010 11:43   doi:10.1186/1471-2121-11-43

Open Data