The N-terminal region (NTR) or tail of RCC1α is required for stable interaction with chromatin in live cells. (A) Schematic diagrams of N-terminal and C-terminal GFP fusion constructs. (B) Representative images of live HeLa cells expressing the N-terminal and C-terminal GFP fusion constructs. (C) Fluorescence recovery after photobleaching (FRAP) of RCC1α-GFP. Images of a cell during the timecourse are shown (left). Circles indicate the region of photobleaching. Output of data (right) with FRAP half-time (t1/2). (D) Mean of the FRAP t1/2 for each GFP fusion construct. Data from 3 independent experiments were combined and tested for significant difference in FRAP t1/2 using the one-way ANOVA test. P-value less than 0.05 was considered to be statistically different (p-value < 0.001 shows as ***).
Hitakomate et al. BMC Cell Biology 2010 11:43 doi:10.1186/1471-2121-11-43