Research article
The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells
1 Biomedical Research Institute, School of Medicine, College of Medicine, Dentistry and Nursing, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
2 The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, UK
BMC Cell Biology 2010, 11:43 doi:10.1186/1471-2121-11-43
Published: 21 June 2010Additional files
Additional file 1:
Figure S1. Fluorescence recovery after photobleaching (FRAP) of RCC1α and mutants fused to GFP at the N-terminus or the C-terminus.
Format: PDF Size: 1.6MB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 2:
Figure S2. Fluorescence recovery after photobleaching (FRAP) showing stabilisation of the interaction of GFP-RCC1α (N-terminal GFP) with chromatin by RanT24N.
Format: PDF Size: 379KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 3:
Figure S3. Deletion of the N-terminal tail and mutation of lysine 4 (K4Q) abolishes methylation of RCC1α. Western blot showing the α-N-dimethylation of RCC1α, Δ27RCC1, RCC1αK4Q, RCC1αD182A and RCC1αS11A.
Format: PDF Size: 294KB Download file
This file can be viewed with: Adobe Acrobat Reader
