Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Research article

The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells

Ekarat Hitakomate1, Fiona E Hood12, Helen S Sanderson1 and Paul R Clarke1*

Author Affiliations

1 Biomedical Research Institute, School of Medicine, College of Medicine, Dentistry and Nursing, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK

2 The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, UK

For all author emails, please log on.

BMC Cell Biology 2010, 11:43  doi:10.1186/1471-2121-11-43

Published: 21 June 2010

Additional files

Additional file 1:

Figure S1. Fluorescence recovery after photobleaching (FRAP) of RCC1α and mutants fused to GFP at the N-terminus or the C-terminus.

Format: PDF Size: 1.6MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Figure S2. Fluorescence recovery after photobleaching (FRAP) showing stabilisation of the interaction of GFP-RCC1α (N-terminal GFP) with chromatin by RanT24N.

Format: PDF Size: 379KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Figure S3. Deletion of the N-terminal tail and mutation of lysine 4 (K4Q) abolishes methylation of RCC1α. Western blot showing the α-N-dimethylation of RCC1α, Δ27RCC1, RCC1αK4Q, RCC1αD182A and RCC1αS11A.

Format: PDF Size: 294KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data