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Multiple mesodermal lineage differentiation of Apodemus sylvaticus embryonic stem cells in vitro

Tao Wang12, Frank Fuxiang Mao12, Wenyu Lai3, Weiqiang Li12, Weihua Yu12, Zifei Wang12, Lirong Zhang5, Jinli Zhang1, Jin Niu6, Xiuming Zhang12, Bruce T Lahn12 and Andy Peng Xiang124*

Author Affiliations

1 Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, 74# Zhongshan Road 2, Guangzhou, 510080, China

2 The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education; Key Laboratory of Stem Cells and Tissue Engineering of Guangdong Higher Education Institutes, Sun Yat-sen University, Guangzhou, China

3 Department of Pediatric, The Second Affiliated Hospital of Sun Yat-sen University, Yanjiang Xi Lu, Guangzhou 510120, China

4 Department of Biochemistry, Zhongshan Medical School, Sun Yat-sen University, Guangzhou, China

5 Department of Pathophysiology, Guangdong College of Pharmacy, Guangzhou, China

6 Bellaire High School, Houston, TX, USA

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BMC Cell Biology 2010, 11:42  doi:10.1186/1471-2121-11-42

Published: 19 June 2010



Embryonic stem (ES) cells have attracted significant attention from researchers around the world because of their ability to undergo indefinite self-renewal and produce derivatives from the three cell lineages, which has enormous value in research and clinical applications. Until now, many ES cell lines of different mammals have been established and studied. In addition, recently, AS-ES1 cells derived from Apodemus sylvaticus were established and identified by our laboratory as a new mammalian ES cell line. Hence further research, in the application of AS-ES1 cells, is warranted.


Herein we report the generation of multiple mesodermal AS-ES1 lineages via embryoid body (EB) formation by the hanging drop method and the addition of particular reagents and factors for induction at the stage of EB attachment. The AS-ES1 cells generated separately in vitro included: adipocytes, osteoblasts, chondrocytes and cardiomyocytes. Histochemical staining, immunofluorescent staining and RT-PCR were carried out to confirm the formation of multiple mesodermal lineage cells.


The appropriate reagents and culture milieu used in mesodermal differentiation of mouse ES cells also guide the differentiation of in vitro AS-ES1 cells into distinct mesoderm-derived cells. This study provides a better understanding of the characteristics of AS-ES1 cells, a new species ES cell line and promotes the use of Apodemus ES cells as a complement to mouse ES cells in future studies.