Additional file 1.
Figure S1: Recombinant constructs. A. Schematic representation of recombinant HOs, NADPH-cytochrome P450 reductase, HRG-1, HCP1, DMT1, and syntaxin 17 showing the locations of transmembrane (TM) domains (blue box) and the order of tags. TM domains were predicted using the TMHMM program. HO-1 and HO-2 have a single TM domain at the C-terminal region, and NADPH-cytochrome P450 reductase has a single TM domain at the N-terminal region. HRG-1, HCP1, DMT1, and syntaxin 17 have multiple TM domains. These proteins are tagged with HA, GFP, or mCherry as depicted. B. GFP-tagged HO-1 and HA-tagged HO-1 were cotransfected in HEp-2 cells and visualized by confocal microscopy. Recombinant construct combinations were N-terminally GFP-tagged HO-1 (a and g), C-terminally GFP-tagged HO-1 (d and j), N-terminally HA-tagged HO-1 (b and k), and C-terminally HA-tagged HO-1 (e and h). C. GFP-tagged HO-1 and HA-tagged HO-2 were cotransfected into HEp-2 cells and stained with antibody against GFP (a) and HA (b). Each inset shows a higher magnification image of the boxed area.
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Yanatori et al. BMC Cell Biology 2010 11:39 doi:10.1186/1471-2121-11-39