Additional file 5.
Time-lapse of embryo development: extended focus projection. Extended focus projection of a mouse embryo imaged with THG (grey) and SHG (magenta). Imaging was started at the compacted morula stage and development followed till the blastocyst stage. Images were captured at 20 minute intervals. The majority of THG signal is generated by lipid droplets, which become fewer and larger over the course of development. Use keyboard arrow keys to navigate frame by frame through the animation. Scale = 20 μm.
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Watanabe et al. BMC Cell Biology 2010 11:38 doi:10.1186/1471-2121-11-38