Additional file 10.
Nuclear stain of control and Nocodazole treated embryos. Opacity renderings of morulae cultured in DMSO (carrier control) and Nocodazole overnight. Embryos were fixed and stained with DAPI to visualise DNA. The control morula (at left) developed normally, and underwent several rounds of division. One can see interphase nuclei as regular spheroids and one cell in metaphase towards the top right of the embryo. In contrast, the nocodazole treated embryo suffered metaphase arrest, as evident from their bumpy appearance due to condensed chromosomes and the presence of only 8 staining bodies.
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Watanabe et al. BMC Cell Biology 2010 11:38 doi:10.1186/1471-2121-11-38