Additional file 1.
Aberration correction improves HGM image quality. (A) Schematic of the adaptive harmonic generation microscope. Lx, lens; Mx, mirror; BSx, beam splitter; DM, deformable mirror; O, objective; S, specimen; C, condenser; D, dichroic; PMTx, photomultiplier tubes for THG (blue) and SHG/TPF (green) signal detection. He-Ne laser (dashed outline) is used for DM characterization and this path is disabled during imaging. (B) Representative THG image of 5.5 dpc mouse embryo, a region of size 30 μm × 30 μm × 15 μm, approximately 90 μm deep in the sample before aberration correction (C) after correcting system induced aberrations. Scale bar is 10 μm. (D) The correction phase function applied to the DM. This consists mainly of spherical aberration, probably due to incomplete coverglass thickness compensation in the objective lens. Compensating system aberrations results in an over all signal improvement of nearly 40%.
Format: JPEG Size: 206KB Download file
Watanabe et al. BMC Cell Biology 2010 11:38 doi:10.1186/1471-2121-11-38