Differentiation increases phosphotyrosine associated p85. Representative western blot of phosphotyrosine associated p85 in C2C12 cells at 3 (subconfluent; SC), 24 (Day 0), 48 (Day 1), or 96 hours (Day 3) following plating. Phosphotyrosine complexes were immunoprecipitated and the associated p85 was determined by western blot and quantified using the IgG HC as a control. * indicates significantly higher than in subconfluent cells.
Hamilton et al. BMC Cell Biology 2010 11:37 doi:10.1186/1471-2121-11-37