S6K1 siRNA knockdown does not affect IRS-1 protein or muscle cell differentiation. A) Representative Blots, B) IRS-1 and S6K1 expression following treatment with siRNA directed against S6K1 and S6K2 for 48hrs post transfection (cells transfected at SC and collected at Day 1), C) IRS-1 and S6K1 levels relative to eEF2 following treatment with siRNA directed against S6K1 and S6K2 for 96 hrs (cells transfected at SC and collected at Day 3), D) Representative images of myotube formation following 96hrs siRNA treatment. Figure headings - SCR = scramble control siRNA, S6K1 = siRNA against S6K1, S6K2 = siRNA against S6K2, S6K1/2 = siRNA against both S6K1&2, SCR+Fl = scramble control plus a fluorescent tagged siRNA, NTC = no treatment control. * indicates significantly differently different from all other treatments.
Hamilton et al. BMC Cell Biology 2010 11:37 doi:10.1186/1471-2121-11-37