Figure 3.

IRS-1/2 expression and associated p85 decrease throughout differentiation. A) IRS-1 and B) IRS-2 total protein was measured via western blots and normalised to relative eEF2 protein content and C) IRS-1 and D) IRS-2 associated p85 was determined as described in methods. All measures were made at 3 (subconfluent; SC), 24 (Day 0), 48 (Day 1), or 96 hours (Day 3) following plating. * indicates significantly different from SC, † indicates significantly different from all other time points, § indicates significantly different from SC and Day 0.

Hamilton et al. BMC Cell Biology 2010 11:37   doi:10.1186/1471-2121-11-37
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