PlGF and VEGF is involved in Angiotensin II-induced proliferation of cultured EA.Hy 926 cells and HUASMCs. Quiescent cells were treated with Ang II (10-6 mol/L), Ang II (10-6 mol/L) and Losartan (10-6 mol/L), Ang II (10-6 mol/L) and the specific neutralizing antibody to PlGF(A) or VEGF (B)(20 μg/mL) for 24 hours, followed by the assessment of cell proliferation by MTT assay. Each value is the mean ± SD of 5 separate determinations. * indicates P < 0.05 vs control cells. # indicates P < 0.05 vs cells treated with Ang II. Ab-PlGF: the specific neutralizing antibody against PlGF. Ab-VEGF: the blocking antibody to VEGF.
Pan et al. BMC Cell Biology 2010 11:36 doi:10.1186/1471-2121-11-36