Figure 2.

Angiotensin II increases VEGF release (A) and VEGFR-1 and -2 mRNA expressions (B, C) in EA.Hy 926 endothelial cells and HUASMCs. (A) Release of VEGF in the cell culture media measured by ELISA. Growth-arrested cells were incubated with Ang II (10-7, 10-6 and 10-5 mol/L) for 8 hrs, 24 hrsor 48 hrs, and VEGF protein released into cell culture media was measured by ELISA (n = 3 in each group). * indicates P < 0.05 vs control EA.Hy 926 cells or HUASMCs. (B) Real-time PCR results: Ang II induced VEGFR-1 mRNA expression in both EA.Hy 926 endothelial cells and HUASMCs. * indicates P < 0.05 vs control EA.Hy 926 cells or HUASMCs. # indicates P < 0.05 vs cells treated with Ang II. (C) Real-time PCR results: Ang II induced VEGFR-2 mRNA expression in both EA.Hy 926 endothelial cells and HUASMCs. * indicates P < 0.05 vs control EA.Hy 926 cells or HUASMCs. # indicates P < 0.05 vs cells treated with Ang II.

Pan et al. BMC Cell Biology 2010 11:36   doi:10.1186/1471-2121-11-36
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