Open Access Research article

Centriole movements in mammalian epithelial cells during cytokinesis

Asta Björk Jonsdottir12*, Roeland W Dirks2, Johannes Vrolijk2, Helga M Ögmundsdottir1, Hans J Tanke2, Jorunn E Eyfjörd1 and Karoly Szuhai2

Author Affiliations

1 Cancer Research Laboratory, Faculty of Medicine, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland

2 Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2300 RC Leiden, the Netherlands

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BMC Cell Biology 2010, 11:34  doi:10.1186/1471-2121-11-34

Published: 21 May 2010

Additional files

Additional file 1:

Centriole tracks in MCF 10A cells during cytokinesis. Centrioles were tracked using the tracking software Stacks (see Figure 1A). Every centriole track is represented by a different color.

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Additional file 2:

Centrosome is mobile in an α-Tubulin net. Centrosome show low mobility in α-Tubulin foci close by the nuclear envelope (inset). Blue-dotted lines represent the nuclear envelope. Images shown are an overlay of centrin1-EGFP (green) and α-Tubulin-mCherry (red).

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Additional file 3:

Centriole mobility in MCF 10A cell during cytokinesis. Representative movie of centriole mobility in epithelial MCF 10A cells during cytokinesis (see Figure 3). Images were collected every 10 minutes using fluorescent excitation and DIC. The centrosome compartments centrioles are marked green and in red are microtubules. Microtubules accumulate and form the intercellular bridge during cytokinesis. A centriole migrates to the intercellular bridge before abscission occurs.

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Additional file 4:

Frequency of centriole repositioning to the intercellular bridge in relation to cell density.

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Additional file 5:

Midbody in extracellular space after abscission. Representative images of release of microtubule particles from the intercellular bridge and fate of midbody after abscission. Intercellular bridge containing the midbody (white arrows) links the two daughter cells. During abscission the bridge is cut. The midbody floats in the extracellular space after abscission.

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