Table 1

Antibodies used in the study.

Antibody

Isotype

Suppliers

Cat. No

Dilution


α-SMA

Mouse IgG2a

Sigma

A2547

1:200

E-cadherin

Mouse IgG2a

BD Biosciences

610182

0.25 μg/ml

TGF-β1

mouse IgG1

R&D Systems

MAB240

5 μg/ml

TβR-I

Rabbit IgG

Cell Signaling Technology

#3712

1:1000

Smad2

Mouse IgG1

Cell Singaling Technology

#3103

1:800

Smad3

Rabbit IgG

Zymed Laboratories

51-1500

1:200

CK-18

Mouse IgG1

Chemicon International

MAB1600

1:200

p-Smad2

Rabbit IgG1

Cell Singaling Technology

#3101

1:200

p-Smad3

Rabbit IgG1

Cell Singaling Technology

#9520S

1:200

Smad3

Rabbit IgG

Epitomics

1735-1

1:2000

p-Smad3

Rabbit IgG

Epitomics

1880-1

1:1000

Smad2

Rabbit IgG

Epitomics

1736-1

1:1000

p-Smad2

Rabbit IgG

Abcam

Ab53100

1:300

Smad7

Rabbit IgG

Santa Cruz Biotechnology

SC-11392

1:100


Wang et al. BMC Cell Biology 2010 11:31   doi:10.1186/1471-2121-11-31

Open Data