Table 1

Expression levels of natural and recombinant bomapin in cells

Cell line

Bomapin (ng bomapin/mg total protein)

HL-60

0.55 ± 0.02

U937

1.85 ± 0.29

THP-1

2.44 ± 0.58

K562 expressing bomapin-EGFP

1.29 ± 0.26

K562 expressing C395S bomapin-EGFP

1.23 ± 0.21

HT1080 expressing bomapin-EGFP

2.58 ± 0.32

Exponentially growing cells were lysed in a lysis buffer containing protease inhibitor cocktail, and bomapin level was quantified by bomapin-specific ELISA (as described in Methods section).

Przygodzka et al. BMC Cell Biology 2010 11:30   doi:10.1186/1471-2121-11-30