Table 1

Fraction of remaining immuno-reactivity

Antigen

Immunoblota

Cytometryb


Mcm6

0.6 ± 0.1 (3)

0.6

Cdc6

0.4 ± 0.0 (2)

0.3

PCNA

0.4 ± 0.1 (2)

0.2


a Optical densities of bands from western blots of lysates from cells that were extracted with Triton X-100 prior to lysis were divided by the optical densities of bands from lysates. Means and standard deviations for multiple experiments (N) are listed.

b Mean fluorescence intensities of Molt4 cells extracted with 0.5% Triton X-100 then fixed with methanol prior to staining were divided by the mean fluorescence intensities of Molt4 cells fixed in methanol then stained.

Frisa and Jacobberger BMC Cell Biology 2010 11:26   doi:10.1186/1471-2121-11-26

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