Additional file 6.
Flow cytometry of Mcm-6 expressed G1 cells. RPE1 cells were grown exponentially (A) or serum starved for 25 h (B). The cells were fixed then stained for Mcm6, PCNA, phospho-S10-histone H3, and DNA. Mitotic cells (blue histogram) were gated on DNA and phospho-S10-histone H3; early S phase cells (red-brown histogram) were gated on DNA and PCNA, and G1 cells (cyan histogram) were gated on DNA and (absence of) PCNA. The Mcm6 frequency distributions from each gate were overlayed to show the relative expression of each. The distributions are plotted with Y scaling set to the frequency peak height, therefore frequencies are normalized (labeled "number"). The exponentially cycling cells are larger and have higher background fluorescence and therefore, broader coefficients of variation (note the broader mitotic and early S distributions). The key information is that the mitotic levels and S phase levels set the bounds of the distribution (min and max) and that the transition state between them is variably populated with slower growing populations shifted to the left and faster growing populations shifted to the right.
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Frisa and Jacobberger BMC Cell Biology 2010 11:26 doi:10.1186/1471-2121-11-26