Isolation and characterization of hADSCs. a, Human ADSCs were isolated from fat tissue according to their adherence to culture dishes. Under phase contrast, hADSCs are spindle-shaped after seven days in culture. Scale bars measure 50 μm. b, Human ADSCs were labeled with FITC-conjugated antibodies specific to CD14, CD34, and CD45. Human ADSCs were labeled with PE-conjugated antibodies specific to CD13, CD44, CD90, and CD166. Surface phenotype was analyzed by FACS. Open histograms refer to control immunoglobulins and colored histograms refer to specific antibodies. Primary hADSCs expressed MSC specific markers including CD13 (+), CD44 (+), CD90 (+), and CD166 (+), but did not express hematopoietic stem cell markers including CD14 (-), CD34 (-), and CD45 (-).
Jang et al. BMC Cell Biology 2010 11:25 doi:10.1186/1471-2121-11-25