Additional file 2.
Comparison of HL-60 and Jurkat leukemic cell lines. A) Co-Immunofluorescence analysis subcellular localization of ID1 and γ-tubulin; nuclei were visualized using Hoechst 33258 (blue). B) Quantitative analysis of cells with n > 2 centrosomes and percent mitotic cells (mean of at least 3 independent experiments, ± standard deviation). C) ID1 protein expression in HL-60 and Jurkat cells. Protein extracts (10 μg) were analyzed by immuno blotting using primary antibodies against ID1 (C-20), and GAPDH (loading control).
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Manthey et al. BMC Cell Biology 2010 11:2 doi:10.1186/1471-2121-11-2