Figure 3.

Assessment of centrosome numbers in established cell lines. Quantitative analysis of centrosome abnormalities and abnormal mitoses depicted as mean of at least 3 independent experiments, bars represent standard deviation. A) Percentage of cells with supernumerary centrosomes in leukemic cells. B) Percentage of cells with supernumerary centrosomes in cervical cancer cells. C) Percentage of cells with supernumerary centrosomes in breast cancer cells. D) Percentage of cells with supernumerary centrosomes in other epithelial cells. E-H: Immunofluorescence analysis. Centrosomes were visualized using γ-tubulin antibody (green); nuclei were visualized using Hoechst 33258 (blue); for each group of cell lines representative examples of normal (left picture) and abnormal (right picture) centrosome numbers are shown; E) Cell lines with a high rate of centrosomal abnormalities, e.g. C33A F) Cell lines with an intermediate rate of centrosomal abnormalities, e.g. MDA-MB468 G) Cell lines with a low rate of centrosome abnormalities, e.g. Kasumi-1 H) Cell lines with a high number of abnormal centrosomes and mitoses, e.g. T47-D.

Manthey et al. BMC Cell Biology 2010 11:2   doi:10.1186/1471-2121-11-2
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