Endogenous ID expression in different cell lines. A) Expression analysis of ID proteins in leukemic cells. B) Expression of ID proteins in cervical cancer cells. C) Expression of ID proteins in breast cancer cells. D) Expression of ID proteins in other epithelial cells. Cells were grown up to 70% confluence and harvested for protein. Protein extracts (10 μg) were then submitted to western blot analysis. Primary antibodies used were anti-ID1, anti-ID2, anti-ID3 and anti-ID4, GAPDH expression as loading control. Examined cell lines and their characteristics are summarized in Additional File 3.
Manthey et al. BMC Cell Biology 2010 11:2 doi:10.1186/1471-2121-11-2