Elevated endogenous expression of the dominant negative basic helix-loop-helix protein ID1 correlates with significant centrosome abnormalities in human tumor cells
1 Department of Hematology and Oncology, University Medical Center Freiburg, Freiburg, Germany
2 Department of Medicine, University Medical Center of Hamburg-Eppendorf, Hamburg, Germany
3 Helmholtz-University Group Molecular Epidemiology, German Cancer Research Center, Heidelberg, Germany
4 Department of Cardiology, University Medical Center Freiburg, Freiburg, Germany
5 Institute of Pathology, University Medical Center Freiburg, Freiburg, Germany
BMC Cell Biology 2010, 11:2 doi:10.1186/1471-2121-11-2Published: 14 January 2010
Additional file 1:
FACS analysis of SiHa, MCF-7, and T47-D cell lines. DNA content of the cells was evaluated using FACS sorting (Dako Cytomation) after cells were treated with RNAse and Propidium iodide. Representative example of A) SiHa, B) MCF-7 and C) T47-D DNA content (64 corresponds to a single set of chromosomes (G1, G0), 128 to a doubled set of chromosomes (G2), counts in between show cells in S phase.
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Additional file 2:
Comparison of HL-60 and Jurkat leukemic cell lines. A) Co-Immunofluorescence analysis subcellular localization of ID1 and γ-tubulin; nuclei were visualized using Hoechst 33258 (blue). B) Quantitative analysis of cells with n > 2 centrosomes and percent mitotic cells (mean of at least 3 independent experiments, ± standard deviation). C) ID1 protein expression in HL-60 and Jurkat cells. Protein extracts (10 μg) were analyzed by immuno blotting using primary antibodies against ID1 (C-20), and GAPDH (loading control).
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