Strong inhibition of adipogenesis in ADSCs by simultaneously applying mechanical strain and adipogenic induction. A. Oil-Red O staining of ADSCs (AM+Strain) that were strained in adipogenic medium for the first 48 h and then by medium alone for the following 12 days. The non-strain cells were adipogenically induced for 14 days. Scale bars = 500 μm. B. AP2 and PPARγ expression in ADSCs (AS) subjected to simultaneous strain for 48 h and adipogenic induction for a total of 5 or 14 days. The non-strained cells (NS) were adipogenically induced for 5 or 14 days **: P < 0.01, *: P < 0.05, N = 3, compared ΔΔCT to NS groups.
Huang et al. BMC Cell Biology 2010 11:18 doi:10.1186/1471-2121-11-18