Expression of osteogenic and adipogenic marker genes in young and old ADSCs. A. Expression of Runx2 and Bglap1 relative to GAPDH in young and old ADSCs after osteogenic induction (Osteo) for 21 days. The marker expression in non-induced cells (Con) is shown. No significant difference was found between young and old ADSCs. N = 6. B. AP2 and PPARγ gexpression relative to β-actin expression in young, adult, and old ADSCs subjected to adipogenic induction (Adipo) for 14 days. The non-induced samples were induced for 21 days as in A. ***: P < 0.001, **: P < 0.01 (N = 3), compared ΔΔCT to young or adult ADSCs.
Huang et al. BMC Cell Biology 2010 11:18 doi:10.1186/1471-2121-11-18