Table 1

Plasmids used in this study

Plasmid

Description

Source


GFP-Mth1

Mth1-GFP fusion protein (pUG34 or pUG36)

17

GFP-NES-Mth1

Mth1-GFP fusion protein with NES

This study

GFP-NES (m)-Mth1

Mth1-GFP fusion protein with NES (L changed to A)

This study

GFP-Grr1

Grr1-GFP fusion protein

This study

GFP-Grr1ΔN

Grr1-GFP fusion protein without amino acids from 1 to 280 [26]

This study

GFP-Yck1

Yck1-GFP fusion protein

This study

GFP-Yck2

Yck2-GFP fusion protein

This study

Mth1 ID 1

GFP-Mth1 fusion protein without amino acids from 1 to 20

This study

Mth1 ID 2

GFP-Mth1 fusion protein without amino acids from 81 to 90

This study

Mth1 ID 3

GFP-Mth1 fusion protein without amino acids from 88 to 119

This study

Mth1 ID 4

GFP-Mth1 fusion protein without amino acids from 118 to 138

This study

Mth1 ID 5

GFP-Mth1 fusion protein without amino acids from 137 to 157

This study

Mth1 ID 6

GFP-Mth1 fusion protein without amino acids from 156 to 180

This study

Mth1 ID 7

GFP-Mth1 fusion protein without amino acids from 179 to 188

This study

Mth1 ID 8

GFP-Mth1 fusion protein without amino acids from 187 to 200

This study

Mth1 ID 9

GFP-Mth1 fusion protein without amino acids from 198 to 214

This study

Mth1 ID 10

GFP-Mth1 fusion protein without amino acids from 213 to 236

This study

Mth1 ID 11

GFP-Mth1 fusion protein without amino acids from 235 to 262

This study

Mth1 ID 12

GFP-Mth1 fusion protein without amino acids from 261 to 299

This study

Mth1 ID 13

GFP-Mth1 fusion protein without amino acids from 298 to 319

This study

Mth1 ID 14

GFP-Mth1 fusion protein without amino acids from 318 to 327

This study

Mth1 ID 15

GFP-Mth1 fusion protein without amino acids from 326 to 343

This study

Mth1 ID 16

GFP-Mth1 fusion protein without amino acids from 342 to 363

This study

Mth1 ID 17

GFP-Mth1 fusion protein without amino acids from 363 to 380

This study

Mth1 ID 18

GFP-Mth1 fusion protein without amino acids from 390 to 433

This study

Mth1 ID 2 tag-less

Mth1 without amino acids from 81 to 90

This study

Mth1 ID 4 tag-less

Mth1 without amino acids from 118 to 138

This study

Mth1 ID 6 tag-less

Mth1 without amino acids from 156 to 180

This study

Mth1 ID 15 tag-less

Mth1 without amino acids from 326 to 343

This study


Pasula et al. BMC Cell Biology 2010 11:17   doi:10.1186/1471-2121-11-17

Open Data