Identification of the functional regions of Mth1 required for glucose-dependent degradation. (A) Mth1 deletion mutants were generated to determine the regions responsible for degradation by successive 10-50 amino-acids deletion analysis. In total, 18 deletions were constructed. (B) The mutant Mth1 proteins containing internal deletions were expressed in the mth1Δ cells (YM6266) and detected by Western blotting using anti-GFP antibody. (C) Four deletion mutants of Mth1 inhibit glucose-induction of HXT1 expression. Four internal deletion constructs without the GFP moiety were coexpressed with the HXT1-lacZ reporter (pBM2636) in the absence (Gal, black bars) and presence (Glu, gray bars) of glucose.
Pasula et al. BMC Cell Biology 2010 11:17 doi:10.1186/1471-2121-11-17