Neither glucose nor the glucose sensors influence the subcellular localization of Mth1. (A) Yeast cells of the indicated genotype expressing GFP-Mth1 were grown to mid-log phase in a selective medium containing 2% galactose. Aliquots were then transferred to 2% galactose medium (Gal) or 4% glucose medium (Glu) and incubated for 60 min. Subcellular localization of GFP-Mth1 was analyzed by fluorescence microscopy. Yeast cells used were: WT (BY4742), rgt2Δsnf3Δ (YM6370), grr1Δ (YM4783), and rgt1Δ (YM4509). (B) Localization of a nondegradable form of Mth1 (Mth1-I85S) in wild-type cells (BY4742). (C) GFP-Rgt1 localization as a positive control.
Pasula et al. BMC Cell Biology 2010 11:17 doi:10.1186/1471-2121-11-17