PDGF treatment increases integrin-linked kinase activity in mouse aortic smooth muscle cell culture. A) Primary mouse aortic SMCs were serum-starved overnight and then treated with 25 ng/ml of PDGF-BB. ILK kinase activity was measured at various timepoints following treatment. PDGF-BB increased ILK kinase activity (two fold increase). SMC culture transfected with ILK siRNA was used as negative control in the assay. As shown in first lane of the blot, ILK expression was completely abolished in negative control group that led to a significant decrease in ILK kinase activity in this group. B) Transfection of mouse aortic SMCs with ILK siRNA for 96 hours completely blocked ILK protein expression. Data presents three independent experiments and is presented as mean ± STDEV (n = 3), where P < 0.05 was considered significant.
Esfandiarei et al. BMC Cell Biology 2010 11:16 doi:10.1186/1471-2121-11-16