Additional file 7.
GFP-R-Ras is endocytosed to lysosomes via a non-caveolin-1 mediated pathway. (A-C) Shown are enlarged images of unfixed cells transfected with GFP-R-Ras constructs that were incubated with 1 μM Lysotracker Red DND-99 for 20 mins followed by washout. All Lysotracker-positive compartments larger than 1.2 μm contained R-Ras (n = 7 GFP-R-Ras(wt) cells, n = 8 GFP-R-Ras(38V) cells). There were numerous, smaller compartments in all cell types with no correlation between GFP-R-Ras and Lysotracker. (D-F) Transfected cells were fixed and stained for phospho-specific (Tyr-14) caveolin-1, but the lack of correspondence with GFP-R-Ras suggests that GFP-R-Ras is endocytosed separate from caveolin-1. Scale bar is 20 μm.
Format: MOV Size: 801KB Download file
Conklin et al. BMC Cell Biology 2010 11:14 doi:10.1186/1471-2121-11-14