Plasma membrane ruffling is dependent on R-Ras. NMuMG cells stably transfected with GFP-β1-integrin (A, a) contained many ruffles (in 23/23 cells imaged) whose retraction from the cell edge can be observed in the accompanying kymograph (lowercase letters, corresponding to each panel). Cells transiently transfected with two different siRNA sequences targeting R-Ras did not have ruffles in 16/16 cells imaged (B, b) even though β1-integrin could be seen at thecell edge in portions of many cells. However kymography analysis of these areas reveals that the β1-integrin is dynamic in cells replete with endogenous R-Ras. (C, c and D, d) GFP-VSVG localizes to the bulk plasma membrane, suggesting that R-Ras has a more general effect on membrane ruffling. Ruffling that was observed in 15/15 cells imaged was abolished in 15/15 cells that were transfected with R-Ras siRNA. Scale bars = 20 μm (A, B, C, D) or 10 mins (a, b, c, d).
Conklin et al. BMC Cell Biology 2010 11:14 doi:10.1186/1471-2121-11-14