β1-integrin traffics through R-Ras-positive vesicles. Time course images of (A) a GFP-R-Ras(wt) transfected Cos7 cell incubated in Alexa Fluor555 (red) labeled β1-integrin antibody solution for 30 mins reveal the interplay of R-Ras and β1-integrin (See Additional file 8, Movie S5). (B, C) The inset of images from the boxed region in A acquired at one minute intervals show that R-Ras and β1-integrin traffic together to large endosomal vesicles. (D) Endocytosed components are then repackaged together and bud off from these organelles. Scale bar is 20 μm.
Conklin et al. BMC Cell Biology 2010 11:14 doi:10.1186/1471-2121-11-14