Additional file 1.
Mitochondrial nuclease activity. A. Purified mitochondria (2 μg protein) from ΔTTHERM_01104910 (a: lane 1) and ΔTTHERM_006222710 (b: lane 2) were incubated with 2 μg substrate plasmid DNA for 30 min at 37°C in 30 μl reaction buffer containing 20 mM KCl and 50 mM MOPS (pH 6.5). Lane 4 and 5 indicate 100-bp ladder size marker and λHindIII-digest, respectively. The substrate DNA appears in the nicked open circular (OC), linear (L), and supercoiled (SC) forms. B. The nuclease assay was performed under various incubation times. Lane 2-4 (a), substrate DNA was coincubated with ΔTTHERM_01104910 mitochondria. Lane 5-7 (b), substrate DNA was coincubated with ΔTTHERM_006222710 mitochondria. Lane 1 shows undigested sample.
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Akematsu and Endoh BMC Cell Biology 2010 11:13 doi:10.1186/1471-2121-11-13