Table 1

Expression and/or phosphorylation activation of proteins in PDL cells subjected to strain denoted in percentage

Molecule

control 0 h

0.25 h

0.5 h

1 h

3 h

6 h


integrin β3 (Int β3)

/

± 0

± 0

± 0

± 0

+32.2% * p < 0.01

focal adhesion kinase (FAK)

/

± 0

± 0

± 0

± 0

+ 28.7% * p < 0.01

phosphorylated focal adhesion kinase (pFAK)

/

+ 12.8% * p < 0.01

± 0

± 0

± 0

± 0

p42/44

/

+ 5.8% *

+ 12.5% * p < 0.01

± 0

- 9.0% * p < 0.01

+ 0.2% *

phosphorylated p42/44

/

+ 2.3% *

- 7.3% *

-10.0% * p < 0.01

+ 0.5% *

+ 10.2% * p < 0.01

phosphorylated p42/44 + inhibitor

/

/

/

/

/

maximum inhibition - 16.0% ⊗ p < 0.01

p38

/

- 5.3% * p < 0.01

- 13.8% * p < 0.01

- 9.6% * p < 0.01

- 3.1% *

+ 26.2% * p < 0.01

phosphorylated p38

/

+ 5.7% p < 0.01

- 1.7%

+ 3.1%

+ 0.9%

+ 11.4% p < 0.01

phosphorylated p38 + inhibitor

/

/

/

/

/

maximum inhibition - 62% ⊗

matrix metalloproteinase 13 (MMP13)

/

+ 8.4% * p < 0.01

+ 1.4% *

+1.53% *

+ 0.7% *

+ 16.0% * p < 0.01

matrix metalloproteinase 13 (MMP13) + pp38 inhibitor

- 11.0% ⊗ p < 0.01

- 17.0% ⊗ p < 0.01

- 18.8% ⊗ p < 0.01

- 13.5% ⊗ p < 0.01

- 12.0% ⊗ p < 0.01

- 25.7% ⊗ p < 0.01

matrix metalloproteinase 13 (MMP13) + pp42/44 inhibitor

- 22.6% ⊗ p < 0.01

- 28.6% ⊗ p < 0.01

- 24.3% ⊗ p < 0.01

- 24.8% ⊗ p < 0.01

- 24.3% ⊗ p < 0.01

-34.1% ⊗ p < 0.01


Densitometric measurements of western blot analysis were calculated in % of relative gray pixel values and normalised to control* or to the respective time point⊗ in the stretch kinetic. Date represent the mean of three independent experiments (n = 3) and means were subjected to the Students T-test. All compared mean values with p < 0.01 were considered as statistical significant.

Ziegler et al. BMC Cell Biology 2010 11:10   doi:10.1186/1471-2121-11-10

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