Figure 9.

K18 modulates E2-promoted cell cycle progression of MCF-7 cells. A, MCF-7 cells were grown in phenol-red free media stripped of steroids for at least 3 days, then transiently cotransfected with the indicated vectors. The pEGFP-N1 plasmid was included to identify transfected cells. After 36 h, cells were treated with or without E2 (100 nM) for an additional 12 h, then labeled with BrdU (10 μM) for 2 h and immunostained for BrdU with a Cy3-conjugated secondary antibody. Cells were assessed for GFP and BrdU, and the proportion of transfected cells positive for BrdU was scored. B, MCF-7 cells were grown in phenol-red free media stripped of steroids for at least 3 days, then transiently cotransfected with the indicated siRNA duplexes. After 36 h, cells were treated with or without E2 (100 nM) for an additional 12 h and assessed for BrdU incorporation as in A. All experiments in A and B were performed in triplicate and were repeated at least 3 times; results are expressed as means ± SEM.

Meng et al. BMC Cell Biology 2009 10:96   doi:10.1186/1471-2121-10-96
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