Figure 6.

Differential expression of K18 regulates the nucleo-cytoplasmic distribution of the endogenous LRP16 protein in MCF-7 cells. A, MCF-7 cells were transiently transfected with Flag-tagged K18. Total, nuclear and cytoplasmic proteins were extracted 48 h after transfection and were subjected to immunoblotting analysis with the indicated antibodies. B, MCF-7 cells were transiently transfected with K18-specific siRNAs or the control siRNA. Total, nuclear and cytoplasmic proteins were extracted 48 h after transfection and were subjected to immunoblotting analysis with the indicated antibodies. β-actin was used as a loading control for total protein extracts and cytoplasmic extracts. Transcription factor Sp1 expressed constitutively in the nucleus was used as a loading control for nuclear protein extracts.

Meng et al. BMC Cell Biology 2009 10:96   doi:10.1186/1471-2121-10-96
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