K18 interacts with LRP16 protein by its C-terminal region mediation. Top panel, schematic illustration of K18 and its mutants. GST-pulldown assays were performed with in vitro-translated [35S]-labeled K18 and its mutants in the presence of GST-LRP16 fusion protein (bottom panel). GST protein was used as a control. K18-C1 was run on a 21% SDS-PAGE gel; the others were run on 12% gels.
Meng et al. BMC Cell Biology 2009 10:96 doi:10.1186/1471-2121-10-96