Relative marker gene expression in MMS outgrowths derived from murine bone marrow (BM; A1), perirenal adipose tissue (PAT; B1), and mediastinal stromal tissue (MST; C1) confirmed chondrogenic differentiation of the mesenchymal progenitor cells. Expression of collagen type II and X is demonstrated by immunostaining (A2, B2, C2; top) and Sox5 as well as Sox6 expression is shown by mRNA fluorescence in situ hybridization (A2, B2, C2; bottom). Nuclei are stained with DAPI (blue). Mean values ± SEM derived from independent samples per experimental group (n = 3) are shown. Bar = 100 μm.
Böhrnsen et al. BMC Cell Biology 2009 10:92 doi:10.1186/1471-2121-10-92