Co-localozation of Nrf1 and MCRS2. 293 (panel a-h) and H1299 cells (panel i-p) were transfected with plasmid constructs expressing either HA-Nrf1 alone (panel a, d, i and l), MCRS2-FLAG alone (panel b, e, j and m), or HA-Nrf1 and MCRS2-FLAG together (panel c, f, g, h, k, n, o and p). After 24 hours, cells were fixed and stained with the antibodies. The antibodies used were the M2 monoclonal antibody against FLAG-tagged MCRS2 and the rabbit polyclonal anti-HA antibody against the HA-tagged Nrf1. The cells were viewed with the phase-contrast microscope (panel a-c and i-k). The green signal (αHA-Nrf1) was obtained with a FITC-conjugated secondary antibody under a confocal microscope (panel d, f, l and n). The red signal (αMCRS2-FLAG) was obtained with a Rhodamin-conjugated secondary antibody under confocal microscope (panel e, g, m and o). The merged images (panel h and p) showed the co-localization of Nrf1 and MCRS2.
Wu et al. BMC Cell Biology 2009 10:9 doi:10.1186/1471-2121-10-9