Interaction of MCRS2 with Nrf1. Left column, GAL4 DNA binding domain (amino acids 1–147) hybrids. Middle column, GAL4 activation domain (amino acids 768–881) hybrids. Right column, yeast colony color after transformation. Minus (-) indicated no yeast colony was noted following growth incubation. A, characterization of the interaction between Nrf1 and MCRS2. Left column, the GAL4 DNA-binding domain (GAL4BD) hybrid. Middle column, the GAL4 DNA-activation domain (GAL4AD) hybrid. Right column, yeast colony color after transformation. The lamin, LEF1ΔN105, and TCF4 constructs in this experiment were used for the negative control. B, The C terminus of Nrf1 was required and sufficient for the interaction with MCRS2. Left column, the various GAL4DB-Nrf1 deletion derivatives. Middle column, GAL4AD-MCRS2. Right column, yeast colony color after transformation. The Nrf1 fragments fused to the GAL4 DNA binding domain are indicated on the left of the diagrams. The relative positions of CNC, basic, and ZIP domains of Nrf1 are also depicted. C, The C terminus of MCRS2 was required and sufficient for the interaction with Nrf1. Left column, GAL4DB-Nrf1 (354–477). Middle column, the various GAL4AD-MCRS2 deletion derivatives, Right column, yeast colony color after transformation. The MCRS2 fragments fused to GAL4 activation domain are shown in the middle of the diagrams. The relative positions of putative ZIP, coiled-coil, FHA domains of MCRS2 are also depicted. D, The photograph of colony formation came from the results of A. The labels of Figure 1D were the same labels of A.
Wu et al. BMC Cell Biology 2009 10:9 doi:10.1186/1471-2121-10-9